Protein Production and Purification (10 ECTS)
Code: TX00EA34-3001
General information
- Enrollment
- 28.11.2022 - 31.12.2022
- Registration for the implementation has ended.
- Timing
- 09.01.2023 - 10.03.2023
- Implementation has ended.
- Number of ECTS credits allocated
- 10 ECTS
- Mode of delivery
- On-campus
- Campus
- Leiritie 1
- Teaching languages
- Finnish
- Degree programmes
- Laboratory Sciences
- Teachers
- Jarmo Palm
- Patrik Paxal
- Tiina Soininen
- Course
- TX00EA34
Learning outcomes
Student can design and conduct protein production from a DNA molecule to a purified and analyzed end product.
Content
- cloning and protein production
- cell culture techniques, basics of stem cell and cancer biology
- protein purification and characterization methods: precipitation, chromatographic and electrophoretic techniques
- combination techniques in characterization of the end product
Prerequisites
Biochemical Analysis
Teaching methods
Lectures
Learning assignments
Laboratory works
Learning materials and recommended literature
Lectures
Alternative completion methods of implementation
-
Internship and working life connections
-
Exam dates and retake possibilities
-
International connections
-
Student workload
Protein production and purification project week ( Week 4 or 5)
Labs of cell culturing, 2x per week
Content scheduling
Protein production and characterization / Tiina Soininen
Cell culturing / Jarmo Palm
Further information for students
-
Assessment methods and criteria
Protein production and characterization 60%
Cell culturing 40%
Evaluation scale
0-5
Assessment criteria, satisfactory (1)
The student is able to:
- apply professional knowledge in the field appropriately
- find information and argue for the use of his sources
- show that he has achieved the targets for core competence
- find out the safety instructions concerning work assignments and to act in accordance with them
Assessment criteria, good (3)
The student is able to:
- use concepts and knowledge in his field fluently and comprehensively
- assess and use information sources and research information with a critical eye
- operate in a wide range of work situations
- act responsibly in work duties, taking safety issues into consideration
Assessment criteria, excellent (5)
The student is able to:
- use theoretical and professional concepts and knowledge like an expert and combine them into larger entities
- apply professional expertise in new situations
- take responsibility for a professional process in its entirety
- be goal-oriented while developing himself/herself and working life
Assessment criteria, approved/failed
The student is able to:
- apply professional knowledge in the field appropriately
- find information and argue for the use of his sources
- show that he has achieved the targets for core competence
- find out the safety instructions concerning work assignments and to act in accordance with them