Study Guide

Objective

This course will teach you how to produce and purify recombinant proteins using various expression systems and techniques. You will learn about the advantages and disadvantages of different vectors and host cells, such as bacteria, yeast, mold, insect cells and mammalian cells. You will also learn about the methods and challenges of expression, fermentation, downstream processing, protein characterization and quality control. You will gain practical skills in cell culture and immunological techniques. Finally, you will apply your knowledge to a recombinant protein production and purification project. This course will prepare you for a career in biotechnology, where you can create innovative products and solutions using recombinant proteins.

Content

1. Cloning and recombinant protein production
2. Different kind of expression vectors and host cells (Bacteria, yeast, mold, insect cells and mammalian cells)
3. Expression methods
4. Basics of fermentation and upstream processes
5. Downstream processes
6. Protein purification and characterization methods, for example: Precipitation, different kind of separation and immunological techniques.
7. Specific issues of biotech products: Legislation, patents, safety, quality, product development and storage.
8. Recombinant protein production and purification project.
9. Cell culture technique in theory and in practice

Prerequisites

Microbiology and Professional English
Gene Technology and Basics in Bioinformatics

Assessment criteria, satisfactory (1)

Demonstrates a basic understanding of expression systems but lacks awareness of their advantages and disadvantages.
Struggles to understand the techniques used in recombinant protein production.
Struggles to acquire practical skills in cell culture.
Has difficulty in developing proficiency in immunological techniques.
Struggles to apply knowledge and skills effectively in the project.

Assessment criteria, good (3)

Grasps the advantages and disadvantages of different expression systems to some extent.
Has a moderate understanding of recombinant protein production techniques.
Gains some practical skills in cell culture but with occasional difficulties.
Attains moderate proficiency in immunological techniques but may face occasional challenges.
Applies knowledge and skills to the project with some challenges.

Assessment criteria, excellent (5)

Excellently comprehends the nuances of various expression systems and their pros and cons.
Demonstrates a thorough understanding of the techniques used in recombinant protein production.
Excellently acquires and demonstrates practical skills in cell culture consistently.
Demonstrates excellent proficiency in immunological techniques consistently.
Excellently applies knowledge and skills to the project, demonstrating a high level of competence consistently.

Assessment criteria, approved/failed

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