Protein Production and Purification (10 cr)

Objective

Student can design and conduct protein production from a DNA molecule to a purified and analyzed end product.

Content

- cloning and protein production
- cell culture techniques, basics of stem cell and cancer biology
- protein purification and characterization methods: precipitation, chromatographic and electrophoretic techniques
- combination techniques in characterization of the end product

Materials

Lectures

Teaching methods

Lectures
Learning assignments
Laboratory works

Employer connections

-

Exam schedules

-

International connections

-

Completion alternatives

-

Student workload

Protein production and purification project week ( Week 6 or 7)
Labs of cell culturing, 2x per week

Content scheduling

Protein production and characterization / Tiina Soininen
Cell culturing / Jarmo Palm

Further information

-

Evaluation scale

0-5

Assessment criteria, satisfactory (1)

The student is able to:
- apply professional knowledge in the field appropriately
- find information and argue for the use of his sources
- show that he has achieved the targets for core competence
- find out the safety instructions concerning work assignments and to act in accordance with them

Assessment criteria, good (3)

The student is able to:
- use concepts and knowledge in his field fluently and comprehensively
- assess and use information sources and research information with a critical eye
- operate in a wide range of work situations
- act responsibly in work duties, taking safety issues into consideration

Assessment criteria, excellent (5)

The student is able to:
- use theoretical and professional concepts and knowledge like an expert and combine them into larger entities
- apply professional expertise in new situations
- take responsibility for a professional process in its entirety
- be goal-oriented while developing himself/herself and working life

Assessment criteria, approved/failed

The student is able to:
- apply professional knowledge in the field appropriately
- find information and argue for the use of his sources
- show that he has achieved the targets for core competence
- find out the safety instructions concerning work assignments and to act in accordance with them

Assessment methods and criteria

Protein production and characterization 60%
Cell culturing 40%

Qualifications

Biochemical Analysis