Study Guide

Objective

The student knows how to isolate, purify and characterize nucleic acids from different materials. S/He understands the basics of DNA cloning and can describe PCR, qPCR and sequencing techniques. The student knows how to make gene editing and understands the steps of recombinant protein preparation.
S/he understands the significance of DNA techniques in various applications, like GMO analyses, food fraud detection, hygiene analyzes of production processes and indoor air quality monitoring. The student is familiar with the Finnish law and regulations and has considered ethical issues regarding gene technology.
The student knows the main molecular biological research methods and is able to apply some of them in practice.

Content

Cloning methods and vector structures in DNA technology. DNA amplification-, hybridization- and sequencing methods as well as RNAi mechanisms and CRISPR/Cas techniques. Gene technology ethics, Finnish legislation and risk analyses.

Examples of various industrial applications of gene technology

Prerequisites

The world of microbes

Assessment criteria, satisfactory (1)

The student is able to:
- search information from a variety of sources
- display learning in professional knowledge and core content in the field
- display learning in the key safety practices in the field

Assessment criteria, good (3)

The student is able to:
- use concepts and knowledge in his field systematically
- find information and assess and use information sources with a critical eye
- apply professional information in professional assignments
- act independently and responsibly in professional contexts
- plan operations with safety in mind

Assessment criteria, excellent (5)

The student is able to:
- use concepts and knowledge in his field professionally
- argue the use of his sources
- argue a course of action on the basis of research information or findings
- to act responsibly, taking safety issues into consideration